Type: GPC Application #: 108250
Catalog#: 719460 - VA 50/4.6 NUCLEOGEL RP 300-8
Detection: UV, 220nm Conditions: a) eluent A: 0.1% trifluoroacetic acid (TFA) in water; eluent B: 0.1% TFA in acetonitrile - water (95 : 5), linear gradient from 20 - 60% B in 22min flow rate 1.5ml/min
b) eluent A: 0.1% TFA in acetonitrile - water (5:95, v/v), eluent B: 0.1% TFA in acetonitrile - water (95:5, v/v), linear gradient 18 - 60% B in 60s flow rate 4ml/min Authors: LC department Source: Macherey-Nagel
Details
Legend: A) Separation of 1. ribonuclease, 2. insulin, 3. cytochrome c, 4. lysozyme, 5.
bovine serum albumin, 6. myoglobin, 7. ovalbumin.
B) Rapid separation of 1. ribonuclease A, 2. cytochrome c, 3. lysoz